2D Western Blots

Ready to screen 2D Western blots from hard to obtain proteins from a range of species and cell types are available. 

The 2D blots use our GenLysate^™, which are prepared using ProteaseArrest^™, a highly efficient protease inhibitor cocktail.  The GenLysate™ are then subsequently treated with Perfect-FOCUS^™ that removes 2D electrophoresis interfering agents and ensures high spot resolution.  The clean GenLysate^™ are then resolved by isoelectric focusing on pH3-10 IPG strips and then 4-20% SDS-PAGE.  The proteins are transferred to PVDF membranes and are supplied ready-to-use for antibody probing. 

The regular 2D blots are 7 x 8cm and can be prepared with any of our GenLysate^™, however please enquire about different size blots and other specific tissues or protein samples.