Bacterial ProteaseArrest
Inhibits serine, cysteine and bacterial specific proteases, including
aminopeptidases, aspartic and cysteine proteases.
Bacterial ProteaseArrest contains agents to
inhibit serine, cysteine, and bacterial specific proteases, including
aminopeptidases, aspartic and cysteine proteases. Bacterial ProteaseArrest contains both irreversible and reversible
protease inhibitors and an optional EDTA solution is provided for inhibition of
metalloproteases.
Due to the optimized
concentration of the various inhibitors, Bacterial
ProteaseArrest shows excellent and specific inhibition of bacterial protease
activities and is therefore suitable for the protection of proteins during
preparation of samples and protein purification from bacteria.
Bacterial ProteaseArrest is supplied with an
optional EDTA solution for the inhibition of metalloproteases and to preserve
the activity of proteins that require divalent cations (Ca2+, Mg2+,
or Mn2+) for their biological activity. Furthermore, EDTA interferes with IMAC
(immobilized metal affinity chromatography), a common purification technique,
so the optional EDTA can be omitted if IMAC is to be used.
Ready-to-use solution: Simply
add an appropriate volume of Bacterial
ProteaseArrest in protein extract or extraction buffer to inhibit protease
activity.
Supplied
as a 100X solution, recommend 1X for final sample volume.
FEATURES
A bacterial specific protease inhibitor cocktail.
Ready-to-Use
Supplied as 100X solution.
APPLICATIONS
- Inhibition
of protease activity in bacterial protein preparations.
- Protection of proteins from proteolysis in
such applications as electrophoresis, purification, storage, assays, and
other applications.
