Genotech - CB Protein Assay™

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G-Biosciences - CB Protein Assay™

CB^TM Protein Assay

A Coomassie Dye Based Protein Assay

An improved Coomassie Dye based protein assay for the simple and rapid estimation of protein concentration.

This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in change of color, the color of Coomassie dye solution changes from brown to blue. The change in color density is proportional to protein concentration. Protein estimation can be performed using as little as 0.5 g protein per assay.

CB-Protein Assay is supplied with a simple to follow protocol. Ready to use reagent that does not require pre-filtering or dilution. Simply mix the protein solution with CB-Protein Dye and read optical density. The protein-dye complexes reach a stable end point in 5 minutes. The CB-Protein Assay is compatible with reducing agents and a wide variety of common laboratory agents listed below

NOTE:- The Coomassie dye based assay is not suitable if the protein solution contains higher than recommended concentration of detergents.

FEATURES

Sensitivity: Linear responses over the range of 0.5µg - 50g protein
Flexible Protocols-Suitable for tube or Titer plate assays
Ready to use assay reagents and no preparation required
Fast color development
Long shelf life, stable for 12 months

APPLICATIONS

Suitable for non-detergent solubilized proteins.
Protein estimation in protein purification, electrophoresis, immuno-analysis, cell biology, molecular biology, and other research applications.
Suitable for protein samples containing common laboratory agents

TOLERANCE TO COMMON LABORATORY AGENTS
The following table lists the agents compatible with the CB-Protein Assay^TM. Table 1 also shows the acceptable concentration of reagents for standard protocols. In most cases, using a correct blank will eliminate or minimize the error caused by interference.

Agents & Compatible Concentration	Agents & Compatible Concentration	Agents & Compatible Concentration
Amino Acids, 1mM	Brij-35, 0.06%	Guanidine-HCl, 6M
Ammonium Sulfate, 1.0M	CHAPS, 0.5%	Glycerol, 10%
Ampholytes, 0.5%	CHAPSO, 0.5%	DMSO, 10%
Barbital	NP-40, 0.5%	Phenol, 5%
Boric acid 1mM	SDS, 0.015%	Methanol, 10%
Citrate, 0.05%	Triton-X100, 0.06	Ethanol, 10%
Deoxycholate, 0.1%	Tween-20, 0.03%	Ascorbic Acid, 50mM
DTT, 1M	HEPES, 0.1M	Glucose, 1M
Cysteine, 10mM	MES, 0.7M	Urea, 3M
2-Mercaptoethanol, 1M	Sodium Azide, 0.5%	Sucrose, 25%
DNA, 1mg/ml	Sodium Chloride, 6M	Sodium Hydroxide, 0.1M
EDTA, 0.1M	Sodium Phosphate, 0.1M	Glycine, 0.1M
EGTA, 0.05M	Tris, 2M	tRNA, 0.35mg/ml

ACCESSORIES

For researchers convenience Genotech offers a wide selection accessories and supplies for protein assays.

Bovine Serum Albumin Standard (Cat.# 786-006) BSA standard (2 mg/ml) prepared in saline buffer. Supplied as 2 x 5 ml size.

Pre-diluted BSA Protein Standards (Cat.# 786-114)
BSA protein standards in an easy-to-use pre-diluted format. The Pre-Diluted Protein Standards allow faster and more reliable protein estimation. The Pre-Diluted Protein Standards are supplied in 6x 5ml aliquots ranging in concentration from 0.1 mg/ml 1.0 mg/ml.
Bovine Gamma Globulin Standard (Cat.# 786-007) γ -Globulin standard (2 mg/ml) prepared in saline buffer. Supplied as 2 x 5 ml size.
Pre-diluted γ -Globulin Protein Standards (Cat.# 786-114G)
Gamma-Globulin protein standards in an easy-to-use pre-diluted format. The Pre-Diluted Protein
Standards allow faster and more reliable protein estimation. The Pre-Diluted Protein Standards are supplied in 6x 5ml aliquots ranging in concentration from 0.1mg/ml 1.0 mg/ml.
Assay Tubes (Cat.# 786-008): Protein assay tubes, 2ml reaction volumes. Allows proper mixing of assay reaction and good color development.
Assay Cuvettes (Cat.# 786-009): Spectrophotometer assay cuvettes, 1ml reaction capacity, supplied as 500 cuvettes per box.

Catalog #	Description	Size	Shipping Condition	Price
786-006	(2mg/ml) Bovine Albumin Standard	2x5 ml	Ambient	US $32.00
786-007	(2mg/ml) Bovine Gamma Globulin Standard	2x5 ml	Ambient	US $32.00
786-008	Assay Tubes, 2 ml reaction volume	500/Box	Ambient	US $59.00
786-009	Assay Cuvettes (1ml)	500/Box	Ambient	US $66.00
786-012	CB-Protein Assay with Albumin Standard	500 Assays	Ambient	US $113.00
786-114	Pre-diluted BSA Protein Standards, (0.1 mg/ml -1.0 mg/ml)	6 x 5 ml	Ambient	US $30.00
786-114G	Pre-diluted γ -Globulin Protein Standards, (0.1 mg/ml -1.0 mg/ml)	6 x 5 ml	Ambient	US $30.00