CHAPS
Type: Zwitterionic detergent
Full Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Mol. Formula: C32H58N2O7S
Mol Weight: 614.9
Form: White solid
Purity: >99%
Solubility: Water soluble
Conductivity: <25µS in a 10% solution
Critical micelle concentration (CMC): 6-10mM (25°C)
Aggregation number: 10
Cloud point: >100°C
Average micellar weight: 6150
APPLICATIONS
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Zwitterionic detergent.
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Non-denaturing.
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Electrically neutral.
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Has all the advantages of sulfobetaine containing detergents: hydrophobic, bile salt, and anionic detergents in a single molecule.
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Better at solubilizing proteins and breaking protein-protein interactions.
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Less protein aggregation than non-ionic detergents.
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Capable of solubilizing opiate receptors.
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CHAPS can be removed from protein solutions with a detergent removing gel or by dialysis.