Carrier Proteins
The use of peptides for the generation of
antibodies against specific peptides has become an essential tool in proteomic
research.
A crucial step in using peptides as antigens is their coupling to carrier
proteins. Peptides are designed to be good epitopes by themselves; however they
fail to elicit a strong antibody response. The reason for this is that the
epitopes are able to bind to the B-cells, but are too small to contain
additional motifs required to associate with class II molecules and T-cell
receptors. The carrier proteins, once coupled to the peptides, provide these
additional motifs and generate a strong antibody response.
The most common carrier proteins are bovine serum albumin (BSA; 67kDa) and
keyhole limpet hemocyanin (KLH; 450-13,000kDa). In addition, we offer cationized
BSA as a carrier protein (HyperCarrierTM). The use of cationized BSA results in a
superior immunogenic response compared to normal BSA.
All three carrier proteins are also offered as activated carrier proteins, named
as ActiveHOOKTM. ActiveHOOKTM carrier proteins have been pre-activated with the
heterobifunctional protein cross-linker sulfoSMCC and therefore are ready for
the addition of a cysteine containing peptides or proteins for a simple one step
reaction.
|
Carrier Proteins |
Activated Carrier Proteins |
|
BSA |
ActiveHOOKTM BSA |
|
KLH |
ActiveHOOKTM KLH |
|
Hyper Carrier TM |
ActiveHOOKTM HyperCarrierTM |
BSA
Bovine serum albumin is a single polypeptide of
67kDa and consists for 59 lysine residues, of which 30-35 have primary amines
that can react with crosslinkers for the coupling of peptides.
FEATURES
- Convenient 10mg vials
- No weighing required
APPLICATIONS
Carrier protein for peptides for the production
of antibodies.
KLH
Keyhole limpet hemocyanin is a widely used
carrier protein due to its large molecular mass (4.5x105-1.3x107kDa
aggregates) and large number of primary amines that can react with
crosslinkers for the coupling of peptides.
FEATURES
Convenient 10mg vials
No weighing required
APPLICATIONS
Carrier protein for peptides for the production
of antibodies.
HyperCarrierTM
HyperCarrierTM is normal BSA that has
been treated with ethylene diamine, which substitutes anionic carboxyl groups
with cationic aminoethyl-amide groups. This cationized form stimulates a
higher immunogenic response, compared to normal BSA, and allows for a greater
concentration of coupled peptides.
FEATURES
Convenient 10mg vials
No weighing required
APPLICATIONS
Carrier protein for peptides for the production
of antibodies.
ActiveHOOKTM BSA
Bovine serum albumin is a single polypeptide of
67kDa and consists for 59 lysine residues, of which 30-35 have primary amines
that can react with crosslinkers for the coupling of peptides. Pre-activated
with sulfoSMCC to bind cysteine containing peptides.
FEATURES
- Convenient 10mg vials
- No weighing required
- Preactivated for simple 1 step reaction
APPLICATIONS
Carrier protein for peptides for the
production of antibodies.
ActiveHOOKTM KLH
Keyhole limpet hemocyanin is a widely used
carrier protein due to its large molecular mass (4.5x105-1.3x107
kDa aggregates) and large number of primary amines that can react with
crosslinkers for the coupling of peptides. Pre-activated with sulfoSMCC to
bind cysteine containing peptides.
FEATURES
- Convenient 10mg vials
- No weighing required
- Preactivated for simple 1 step reaction
APPLICATIONS
Carrier protein for peptides for the
production of antibodies.
ActiveHOOKTM
HyperCarrierTM HyperCarrierTM is normal BSA that has been treated with ethylene diamine, which substitutes anionic
carboxyl groups with cationic aminoethyl-amide groups. This cationized form
stimulates a higher immunogenic response, compared to normal BSA, and allows for
a greater concentration of coupled peptides. Preactivated with sulfoSMCC to bind
cysteine containing peptides.
FEATURES
- Convenient 10mg vials
- No weighing required
- Pre-activated for simple 1 step
reaction
APPLICATIONS
Carrier protein for peptides for the
production of antibodies.
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