CasPASE™ Apoptosis Assays

Protocol MSDS Certificate of Analysis

Catalog Description Size Price Action Shipping Condition Files
786-200A CasPASE™ 1, 4, 5 assay with Ac-WEHD- AFC substrate 50 assays $200.00 Blue Ice
786-200B CasPASE™ 1, 4, 5 assay with Ac-WEHD-AFC substrate 100 assays $265.00 Blue Ice
786-206A CasPASE™ 13 assay with Ac-LEED-AFC substrate 50 assays $200.00 Blue Ice
786-206B CasPASE™ 13 assay with Ac-LEED-AFC substrate 100 assays $265.00 Blue Ice
786-201A CasPASE™ 2 assay with Ac-VDVAD-AFC substrate 50 assays $200.00 Blue Ice
786-201B CasPASE™ 2 assay with Ac-VDVAD-AFC substrate 100 assays $265.00 Blue Ice
786-202A CasPASE™ 3, 7, 10 assay with Ac-DEVD-AFC substrate 50 assays $200.00 Blue Ice
786-202B CasPASE™ 3, 7, 10 assay with Ac-DEVD-AFC substrate 100 assays $265.00 Blue Ice
786-203A CasPASE™ 6 assay with Ac-VEID-AFC substrate 50 assays $200.00 Blue Ice
786-203B CasPASE™ 6 assay with Ac-VEID-AFC substrate 100 assays $265.00 Blue Ice
786-204A CasPASE™ 8 assay with Ac-LETD-AFC substrate 50 assays $200.00 Blue Ice
786-204B CasPASE™ 8 assay with Ac-LETD-AFC substrate 100 assays $265.00 Blue Ice
786-205A CasPASE™ 9 assay with Ac-LEHD-AFC substrate 50 assays $200.00 Blue Ice
786-205B CasPASE™ 9 assay with Ac-LEHD-AFC substrate 100 assays $265.00 Blue Ice

The CasPASE^™ Apoptosis assays are designed to monitor apoptosis by measuring caspase activity, an early indicator of apoptosis. The assay can be monitored with a fluorescence reader, spectrophotometer, or a titer plate reader.

The kit uses AFC-substrate (7-amino-4-trifluromethyl coumarin conjugated at C-terminal), which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC). The AFC substrate is both chromogenic (yellow-green color visible to the naked eyes) and fluorogenic (detected at 510-550nm with a fluorometer). During the reaction, the substrate releases AFC free dye and undergoes both a fluorescence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value.

When measuring fluorescence, the assay reaction is excited at 380-400nm and emission is read at 510-550nm. If the assay is measured with a spectrophotometer or a titer plate reader, the absorbance is measured at 380-400nm.

The assay can be conveniently adapted for high-throughput 96-well format. The assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.

CasPASE^™ assays are available for caspase enzymes 1-10 and 13. Each CasPASE^™ assay kit is supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and the potent caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards. Available in a 50 or 100 assay size.

Additional Images

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FEATURES

Assays for caspase enzymes 1-10 and 13 available
Exhibits both fluorescence & absorbance spectral shifts
All kits supplied with enzyme specific AFC-substrate, free dye (AFC) & inhibitor Z-VAD-FMK

APPLICATIONS

Assay of apoptosis in cells and tissues.
Characterization of caspase enzymes.

GbioSciences

GbioSciences

CasPASE™ Apoptosis Assays

GbioSciences

GbioSciences

CasPASE™ Apoptosis Assays

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