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DNA Clean Up & Concentration

Download the Molecular Biology Handbook for Complete Product Listing

 

G-Biosciences offers several unique products for DNA clean up, PCR clean up and the clean up and concentration of nucleic acids. The kits use an array of techniques, including dialysis, resin binding and gel filtration to remove unwanted reagents from the nucleic acid samples. For DNA concentration samples, the use of resin binding columns and gel electrophoresis with subsequent gel extraction can concentrate nucleic acid samples.

DNA Clean Up

Genomic Tube-O-DIALYZER™ rapidly dialyzes >100kb genomic DNA samples to remove small DNA, salts, RNA and other contaminants with minimal hands-on manipulation preserving the quality of large DNA templates. 

GET™ CLEAN DNA kit uses our high binding affinity GET™ Spin Columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100bp to >20kb. GET™ Spin Columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.

Our range of SpinOUT™ columns feature:

  • SpinOUT™ For PCR for PCR Clean Up to remove salts, radioisotopes, dyes, primers and deoxynucleotides (dNTPs) from PCR products
  • SpinOUT™ GT-600 and SpinOUT™ GT-1200, are ideal for removing excess unincorporated dye terminators, freeing nucleotides from sequencing and labeling reactions. The are a range of sized gel filtration columns for the purification and clean up of DNA fragments >10 bases (GT-600) and 20 bases (GT-1200)
  • The SpinOUT™ G-Acryl gel filtration columns are acrylamide based resins and are suitable for purification and clean up of DNA fragments >10 bases (G-Acryl 600) and 20 bases (G-Acryl-1200)

DNA Gel Extraction

G-CAPSULE™ is a simple electroelution device that excises DNA or nucleic acid bands and elutes your sample in a final volume of ~30µl. No requirement for heating or additional chemicals.

GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Gene/Protein:
Electroelution of nucleic acids and proteins has many advantages as it avoids centrifugation, vortexing, heating, precipitation and allows minimal manipulation of samples. Electroelution normally involves dialysis tubing, which results in extreme dilution of precious samples. G-Capsule™ is a s..
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Gene/Protein:
Genomic Tube-O-DIALYZER™ rapidly dialyzes >100kb genomic DNA samples to remove small DNA, salts, RNA and other contaminants with minimal hands-on manipulation.   Based on our patented Tube-O-DIALYZER™, the genomic Tube-O-DIALYZER™ has a dialysis membrane secured in th..
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This kit is based on our GET™ Spin Columns, spin columns with a high binding affinity for DNA.      GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, ..
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Gene/Protein:
GET™ CLEAN DNA kit uses our high binding affinity GET™ Spin Columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100bp to >20kb. GET™ Spin Columns has an enhanced binding affinity for DNA, thus elimina..
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The SpinOUT™ PCR columns are spin format desalting columns that have the ability to remove salts, radioisotpes, dyes, primers and  deoxynucleotides (dNTPs) for nucleic acids following PCR.  Two sizes are available:   SpinOUT™ PCR-20: For rapid purification of PCR ..
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Gene/Protein:
Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.    The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin-format columns for the desalting and buffer exchange of peptide, prot..
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The SpinOUT™ G-Acryl columns are versatile, spin-format columns for the desalting and buffer exchange of peptide and protein and other macromolecule solutions ranging from 5µl through to 3ml sample volumes.  The SpinOUT™ columns are available in three MWCO sizes for >1,800,..
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