DetergentOUT™ DTG-100X

  • Protocol, Manual or Handbook Protocol MSDS, Material safety data sheet MSDS CofA, Certificate of Analysis Certificate of Analysis
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  • 786-152

  • DetergentOUT™ DTG-100X, Micro

  • 10
  • $105.00
  •   Add to Cart
  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet
  • 786-153

  • DetergentOUT™ DTG-100X, Medi

  • 10
  • $205.00
  •   Add to Cart
  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet

A one step, column based system for removing free detergents from protein solutions in under 10 minutes. Protein solutions containing detergents are loaded onto a DetergentOUT™ DTG-100X column and spun to elute the detergent free protein solution. Protein recovery is generally >95%, however, when used for highly hydrophobic proteins, depending on their hydrophobicity, some protein may be lost due to the interaction with the column matrix. For removal of detergents from hydrophobic proteins, use of OrgoSol DetergentOUT™ (Cat. # 786-127, 786-128) is recommended.
 
The DetergentOUT™ DTG-100X is suitable for a wide variety of detergents, including Triton® X-100, Nonidet® P-40, Tween® 20, CTAB, CHAPS, and deoxycholate from protein solutions.
 
The kit is available as a:
  • Micro Kit for processing up to 0.5ml protein solution/ column
  • Medi Kit for processing up to 2ml protein solution/ column
Additional Images

  • figure-1
APPLICATIONS

    For the removal of free detergent from protein solutions

References

    Removal of Triton® X-100 during the polymerization of lipid II into peptidoglycan (1, 2) and in the isolation of basolateral membranes from rat AT2 cells (3).

    1. Higgins, D.L. et al (2005) Antimicrob. Agents Chemother. 49: 1127-34.
    2. Baizman, E.R., (2000) Microbiology. 146: 3129-40.
    3. Fisher, J.L. and Margulies, S.S. (2002) 283: L737-46