A one step, column based system for removing free SDS (sodium dodecyl sulfate) from protein solutions in under 10 minutes. Protein solutions containing SDS are loaded onto a DetergentOUT™ SDS-300 column and spun to elute the SDS free protein solution. Protein recovery is generally >95%, however, when used for highly hydrophobic proteins, depending on their hydrophobicity, some protein may be lost due to the interaction with the column matrix. For removal of detergents from hydrophobic proteins, use of OrgoSol DetergentOUT™ (Cat. # 786-127, 786-128) is recommended.
The DetergentOUT™ SDS-300 is supplied with a SDS detection and estimation kit to determine the efficiency of SDS removal.
The kit is available as a:
Additional Images
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APPLICATIONS
- For the removal of free SDS from protein solutions
References
Removal of SDS from human milk used to inactivate HIV type 1 (1) and from alveolar cell homogenates prior to protein concentration determination (2),
- Hartmann, S.U., (2006) J. Hum. Lact. 22: 61-74.
- Cavanaugh, K.J., et al. (2001) Am. J. Respir. Cell Mol. Biol. 25: 584-91.