A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilized on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction.
In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilized in research laboratories.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
FEATURES & APPLICATIONS
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Teaches antibody:antigen precipitation.
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Hands-on activity to precipitate antigen and antibody complex.
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Quantitative assessment of antibody concentration.
ADDITIONAL HARDWARE
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Low speed centrifuge for 1.5-2ml tubes.
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Rotator to hold 1.5ml tubes or a Shaking Incubator, UV light box.