Plasmid Isolation (Solid Particle)

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  • BE-311
  • Plasmid Isolation (Solid Particle)
  • 6 groups of 4-5 students
  • $103.00
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  • Protocol, Manual or Handbook

Bacterial plasmids, the non-genomic transferable DNA, can easily be purified from bacteria using numerous techniques.  The purification of DNA is important for genetic research as it provides a source of transferable DNA and allows researchers to isolate large amounts of recombinant DNA. A second commonly used technique for plasmid isolation, following lysis of bacteria, is when the DNA is immobilized on a glass particle support, where it can be washed free of contaminants and then the purified DNA is eluted.
Students learn an alternative technique for plasmid purification, utilizing a DNA binding solid support to purify the DNA from a bacterial lysis. 
 
 
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
FEATURES & APPLICATIONS
    • Teaches bacterial lysis.
    • Teaches solid phase DNA purification.
ADDITIONAL HARDWARE
    • Shaking Incubator
    • Waterbath or beaker and thermometer
    • Agarose Electrophoresis Equipment
    • Low speed centrifuge for 1.5-2ml tubes.