Supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible with 2D gel electrophoresis. 
FOCUS™ Cytoplasmic & Nuclear proteins fractionation kit is based on the Nuclear & Cytoplasmic Extraction Kit, however it has been modified to be fully compatible with 2D electrophoresis and subsequent downstream processes.
The kit is provided with reagents necessary for fractionation of cytoplasmic and nuclear proteins as well as solubilization buffer suitable for IEF/2D gels for better spot resolution.
 

This kit is designed for >50 preps, depending on the tissue used, where one prep is 20x10⁶ mammalian cells or 100mg mammalian tissue.