Isolates high quality plasmid DNA from 1-5ml E. coli cultures. The kit utilizes an enhanced DNA binding column to produce high yields of plasmid. This quick and easy protocol eliminates toxic phenol/ chloroform extractions or lengthy ethanol precipitations. On completion of the protocol, the plasmid DNA is ready for restriction enzyme digestion, sequencing, subcloning and in vitro transcription. Plasmid yields are typically up to 20µg/prep.