GET™ Plasmid DNA Mediprep isolates super-coiled plasmid DNA from 25-200ml
E. coli cultures. The extraction method is based on alkaline lysis of bacteria and uses our high affinity
GET™ Plasmid Medi Columns to bind and purify plasmid DNA.
The extracted plasmid is ready for further DNA manipulation, such as restriction enzyme digestion, ligation, transformation, transfection and sequencing.
The yield is on average 20-100µg/ prep depending on the volume of bacteria and plasmid copy number.
FEATURES
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For 25-200ml bacterial cultures
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Isolate up to 0.1mg ready-to-use plasmid DNA
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No toxic chemicals, no phenol, no hazardous waste
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Available as 25 or 50 preps
APPLICATIONS
- For plasmid isolation from large bacteria cultures