Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8mg GST/ml resin. Supplied as slurry in 20% ethanol.

 

 The resin, columns and binding/wash and elution buffers are available separately.

 

Our new HOOK™ GST Protein Purification kits are now available and include everything required for purification of GST tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. Kits available are:

HOOK™ GST Protein Purification (Bacteria): Isolation from bacteria; optimized to yield up to 10mg/250ml culture of soluble GST tagged protein.

HOOK™ GST Protein Spin Purification (Bacteria): Isolation from bacteria;optimized to yield ~1mg/50ml culture of soluble GST tagged protein

HOOK™ GST Protein Purification (Yeast): Isolation from yeast; optimized to yield up to 10mg of soluble GST tagged protein.

HOOK™ GST Protein Spin Purification (Yeast): Isolation from yeast; optimized to yield up to 1mg of soluble GST tagged protein.