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HOOK™-NHS-Biotin


HOOK™ NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.

 

For our complete HOOK™ NHS-Biotin labeling kit, click here.

 


Features

  • Synonyms:  N-Succinimidyl D-biotinate, succinimidyl biotin
  • CAS Number:  35013-72-0
  • Linear Formula:  C14H19N3O5S
  • Molecular Weight:  341.38
  • Spacer Arm (Å):  13.5
  • Reactive Group:  NHS-ester
  • Membrane Permeable:  Yes
  • Water Soluble: No
  • Cleavable/ Reversible:  No
  • Reaction pH:  7-9

 

Applications

  • For labeling protein amine groups with NHS-Biotin

 

 

Protocol
786-083
BG-01
Material Safety Data Sheet
786-083
English_US
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BG-01
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
NHS Ester Reactivity Assay
Protein Labeling & Conjugation

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