HOOKTM Peptide Coupling Kit
The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research.
A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response.
The most common carrier proteins are bovine serum albumin (BSA; 67kDa) and keyhole limpet hemocyanin (KLH; 450-13,000kDa). We offer both products in single-use OneQuantTM format. In addition, we offer cationized BSA as a carrier protein. The use of cationized BSA results in a superior immunogenic response compared to normal BSA.
We offer two HOOKTM Peptide coupling kits that allow researchers to attach peptides to their carrier of choice using either primary amines or sulfhydryls in the peptide. Both kits can be supplied with or without BSA, KLH and HyperCarrier proteins.
HOOKTM Peptide Coupling Kit (Amine Reactive)
This kit is designed for the coupling of peptides to carrier proteins, utilizing the primary amines and carboxyl groups of the peptides and carrier proteins. This kit utilizes the chemical heterobifunctional crosslinker EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) to couple peptides to carrier proteins. EDC first reacts with the carboxyl groups, producing an amine reactive intermediate, O-acylisourea that rapidly reacts with the amine groups of the peptide/protein.
This kit utilizes Genotechs Tube-O-ReactorTM, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands on time and effort.
FEATURES
- Simple to use, single step reaction
- Suitable for all carrier proteins
- Utilizes Tube-O-ReactorTM for minimum hands on time
APPLICATIONS
- Coupling of peptides to carrier proteins.
- Suitable for antibody production.
HOOKTM Peptide Coupling Kit (Sulfhydryl Reactive)
A common practice in the design of peptides is the addition of a cysteine residue at the N- or C-terminus of the peptide, which can be used for coupling to carrier proteins. This kit is designed for the coupling of peptides to carrier proteins, utilizing a sulfhydryl group in the peptide. This kit exploits the chemical crosslinker sulfoSMCC (Sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) to couple peptides through their sulfhydryl groups, found on cysteine side chains, to the primary amines on the carrier proteins. The N-hydroxysuccinimide (NHS) ester in sulfoSMCC reacts with primary amines to form covalent amide bonds and the maleimide group reacts with sulfhydryl groups to form stable thioether bonds.
If peptides do not contain a sulfhydryl group then Genotech recommends and supplies Trauts reagent (2-IminothiolaneHCl). Trauts reagent modifies primary amines, located at the N-terminus and on lysine side chains, introducing a sulfhydryl group that is fully compatible with this kit.
This kit utilizes Genotechs Tube-O-ReactorTM, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands on time and effort.
FEATURES
- Simple to use
- Contains reagents to reduce peptides
- Suitable for all carrier proteins
- Utilizes Tube-O-ReactorTM for minimum hands on time
APPLICATIONS
- Coupling of peptides to carrier proteins.
- Suitable for antibody production.
