HOOK™ BiotinQuant

HOOK™ BiotinQuant measures biotin using HABA [4'-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site. A characteristic color, that absorbs at 500nm, is produced (e=35,500 M^-1 cm^-1 expressed as per mole of HABA bound). Biotin or biotinylated agents compete with the HABA for the binding sites and the greater affinity biotin reagents displace HABA from the avidin binding sites and proportionally reduce the absorbance.