LabSafe GEL Blue™ is an enhanced protein stain that is based on Coomassie dye that offers unsurpassed sensitivity and rapid band visualization. LabSafe GEL Blue™ is supplied in a ready-to-use format, which is added directly to protein gels following electrophoresis after a brief wash step.
LabSafe GEL Blue™ is a sensitive stain that is able to detect as little as 4ng protein (see figure 1 below). Protein bands are visible in as little as 3 minutes, with maximal staining achieved in an hour (see figure). The figure shows that LabSafe GEL Blue™ can detect protein levels as low as 4ng BSA. 60-1000ng BSA is detectable in 5-10 minutes and the low levels of BSA (4-8ng) become clearly visible when washed in water.
Additional Images
figure-1
FEATURES
- Sensitive, 4-8ng BSA.
- Saves time, band develops within 3 minutes.
- Avoids the use of methanol, acetic acid, or other toxic agents.
- Works with native PAGE, SDS-PAGE, isoelectric focusing, or 2D gels.
APPLICATIONS
- Staining of protein electrophoresis gels.
- Suitable for native, denaturing, isoelectric focusing, and 2D gels.
References
- Taylor, R.M., et al (2006) J Biol. Chem. 281:37045.
- Stie, J., et al (2007) J Leukoc. Biol. 82:161.