Newsletter

Enter email address to receive monthly newsletter and discounts


OmniPrep™


The OmniPrep™ kit isolates high quality genomic DNA from many different species and tissue types including animal, plant, bacteria, yeast, fungi, whole blood, and cells in culture. DNA can be isolated from samples high in polysaccharides or other contaminants that are difficult to remove from the DNA preparations.

The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. Pure genomic DNA is isolated in 20-40 minutes, depending on the tissue sample type used. The resulting genomic DNA is visualized on an agarose gel as a large single band, demonstrating the high quality and minimal shearing of the technique.

OmniPrep™ isolates genomic DNA from a wide range of tissues and samples, including:

  • Mammalian tissues (fresh or frozen)


 

  • Cultured cells


 

  • Paraffin embedded tissues


 

  • Ethanol or formalin fixed tissues


 

  • Nucleated blood cells from bird, fish and frog


 

  • Bacteria (Gram positive and negative)


 

  • Plant tissues (fresh or frozen, rich in polysaccharides)


 

  • Mouse tail


 

  • Yeast


 

  • Fungi


 

  • Blood


 

  • Blood stained and bodily fluid stained material


The OmniPrep™ protocol contains individual protocols for the different samples and instructions on how to adapt for large sample volumes.

Additional Images

  • figure-1
FEATURES
    • High Yield, ~100kb genomic DNA.
    • A260/A280 between 1.8 and 2.0.
    • Adaptable for variety of samples, including plant, bacteria, fungi, mammalian tissues, cells and blood.
    • Simple two tube method, 20-40 minutes.
    • No toxic chemicals, no phenol, no hazardous waste.
    • Suitable for ~150 preparations depending on sample.
APPLICATIONS
    • Extraction of pure genomic DNA from cells and tissues, plant, fungi, bacteria, whole blood, and other samples.
    • Extraction of high quality, 100kb genomic DNA.
References
    • OmniPrep

    1. Choi, Y. et al (2008) Mycologia 100: 701-09.

    • Isolated from plant leaves
      1. Li et al (2002) Genome 45:229.
      2. Lee et al (2005) Genome 48:1104.

     

    • Isolated from murine brain and liver
      1. Pliss et al (2004) J. Neurochem. 91:1082.

     

    • Isolated from gram negative bacteria
      1. Villar et al (2001) J. Bacteriol. 183:55.

     

    • Isolated from erythoid cell lines
      1. Jacobs-Helber et al (2002) J. Biol. Chem. 277:4859.
MSDS   
  • Catalog
  • Description
  • Size
  • Price
  • Action
  • Shipping Condition
  • Protocol