Based on our popular OmniPrep™ system, the OmniPrep™ for Yeast kit isolates high quality genomic DNA from yeast. The kit isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA (~100kbp) and the yield is 3-6μg/ml yeast culture. If used according to the protocols this kit purifies DNA from 300ml yeast culture.
The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA. This kit is supplied with a proprietary Yeast Suspension Buffer and LongLife™ Zymolyase® for the rapid resuspension and digestion of the yeast cell walls for DNA release. Pure genomic DNA is isolated in 20-40 minutes, depending on the tissue type used.
FEATURES
- High Yield, ~100kb genomic DNA.
- A260/A280 between 1.8 and 2.0.
- Simple two tube method, 20-40 minutes.
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Supplied with Yeast Suspension Buffer and LongLife™ Zymolyase® for efficient DNA release.
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No toxic chemicals, no phenol, no hazardous waste.
APPLICATIONS
- Extraction of pure genomic DNA from yeast tissue.
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Extraction of high quality, 100kb genomic DNA.