Based on our popular OmniPrep™ system, the OmniPrep™ for Fungi kit isolates high quality genomic DNA from fungal samples. The kit isolates high purity (A260/A280 ratios of 1.8 to 2.0) DNA (~100kbp) and the yield is 0.2-1µg/5mg fungal samples. If used according to the protocols this kit purifies DNA from 1-2gm fungal tissues.
The kit uses a rapid precipitation technique that uses unique precipitation reagents to isolate genomic DNA free from proteins and RNA.
This kit is supplied with Molecular Grinding Resin™ for the rapid release of DNA from the fungal tissue. Pure genomic DNA is isolated in 20-40 minutes, depending on the tissue type used.
FEATURES
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High Yield, ~100kb genomic DNA.
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A260/A280 between 1.8 and 2.0.
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Simple two tube method, 20-40 minutes.
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Supplied with Molecular Grinding Resin™ for efficient fungal disruption.
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No toxic chemicals, no phenol, no hazardous waste.
APPLICATIONS
References
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Szabo, L. J. (2007) Mol. Ecol. Notes. 7: 92
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Ordonez, M. E. and Kolmer, J. A. (2007) Phytopathology. 97: 574
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Sagaram, U. S. et al (2006) Mol. Plant Pathol. 7: 381
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Mertens, J. A. et al (2006) Arch. Microbiol. 186: 41