(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.

TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.

 The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond. Soluble in DMF and acetonitrile.

FEATURES

• Single-use aliquots

• No weighing required

• Ideal quantity for single reactions

APPLICATIONS

• Labeling of peptides, proteins, nucleic acids and nucleotides with a red fluorescent dye.

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ONEQUANT^TM FITC

FITC (fluorescein isothiocyanate) is probably one of the simplest and commonly used fluorescent labels for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.

FEATURES

• Single-use aliquots
• No weighing required
• Ideal quantity for single reactions
   

APPLICATIONS

• Labeling of peptides and proteins with a green fluorescent dye.

ONEQUANT^TM NHS-Biotin

Primary amines, located in lysine side chains and the N-terminus, are highly abundant groups in proteins that are used for the conjugation of labels. NHS-Biotin utilizes the N-hydroxysuccinimidyl ester group to couple biotin to proteins via their primary amines, forming a stable amide bond.
Soluble in DMSO or DMF, Spacer Arm: 13.5 , Reaction pH: 7-9, Membrane Permeable
 

FEATURES

• Single-use aliquots
• No weighing required
• Ideal quantity for single reactions
   

APPLICATIONS

• Coupling of a biotin label to peptides and proteins.

ONEQUANT^TM sulfo-NHS-LC-Biotin

Primary amines, located in lysine side chains and the N-terminus, are a highly abundant groups in proteins that are used for the conjugation of labels. Sulfo-NHS-LC-Biotin utilizes the N-hydroxysuccinimidyl ester group to couple biotin to proteins via their primary amines, forming a stable amide bond. Unlike NHS-Biotin, it is water soluble up to ~10mM and contains an larger spacer arm. Soluble in water, Spacer Arm: 22.4, Reaction pH: 7-9 Membrane impermeable.
 

FEATURES

• Single-use aliquots
• No weighing required
• Ideal quantity for single reactions
   

APPLICATIONS

• Coupling of a biotin label to peptides and proteins

ONEQUANT^TM Iodoacetyl-LC-Biotin

Iodoacetyl-LC-Biotin is a sulfhydryl reactive reagent that only reacts with free sulfhydryl (-SH or thiol) groups. Cysteine is the most common source of a sulfhydryl group. If free sulfhydryl is not available, they can be generated by reduction of disulfides (-S-S-). Reduction of disulfides is generally performed using a reducing agent such as DTT.
The iodoacetyl group primarily reacts with sulfhydryl groups at pH7.5-8.5, forming a stable thioether bond.
Spacer Arm: 27.1 . Reaction pH: 7.5-8.5, Membrane Permeable, Solvent: DMF or DMSO
 

FEATURES

• Single-use aliquots
• No weighing required
• Ideal quantity for single reactions
   

APPLICATIONS

• Coupling of a biotin label to peptides and proteins.