For researchers convenience we supply buffers that provide the optimum conditions for specific protein reactions, including protein labeling, modification, and cross-reaction.

The Optimizer Buffers are designed for a specific group of chemical reactions.
Optimizer Buffer -I is free of amines and is specific for amine reactive groups, such as imidoesters and N-hydroxysuccinimide (NHS) esters.

Optimizer Buffer -II has a high pH and is specifically designed for the sulfhydryl reactive haloacetyls, such as iodoacetyl.

Optimizer Buffer -III is designed for the sulfhydryl reactive groups, maleimides and pyridyl disulfides, and has reagents that prevent oxidation of free sulfhydryls.

Optimizer Buffer -IV is designed to provide the optimal conditions necessary for carboxyl reactive reagents.
 
Optimizer Buffer V is a modified acetate buffer that is specific for carbohydrate reactive groups.

Optimizer Buffer VI is an alkaline buffer specific for the formation of Schiffs base between amines and glyoxal groups.

These buffers contain optimized concentration of buffering agents, pH, and other co-factors for specific reactions. Simply exchange the buffer of your sample with a suitable Optimizer Buffer and you are ready for efficient reaction. Use of Tube-O-Reactor is recommended for buffer exchange and best reaction results. For additional information on Tube-O-Reactor, click Tube-O-Reactor