PEROXsay™ Peroxide Assays

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  • 786-441
  • PEROXsay™-LIPID

  • 250 assays
  • $73.00
  •   Add to Cart
  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet
  • 786-440
  • PEROXsay™ assay

  • 250 assays
  • $72.00
  •   Add to Cart
  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROXsay™ assay allows the ferric (Fe3+) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
 
PEROXsay™ is suitable for quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.
 
The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.
 
PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.
Additional Images

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FEATURES
    • Colorimetric Assay
    • Linear Range: 0-50µM
    • Highly Reproducible
    • Enzyme Independent
    • Rapid, Accurate & Inexpensive
APPLICATIONS
    • Assay peroxide concentrations in biological solutions
    • Measure lipid peroxidation
    • Monitor protein glycation
References
    1. Bancells, C. et al (2009) J. Lipid Res. 50: 446-455

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