PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the PEROXsay™ assay allows the ferric (Fe3+) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
PEROXsay™ is suitable for quantifying the level of protein damaging peroxides in biological solutions, measurement of lipid peroxidation of low density lipoproteins and liposomes and monitoring protein glycation.
The PEROXsay™ assay is designed for microtiter plates, but can be scaled up for use with 1ml cuvettes.
PEROXsay™-LIPID permits the quantification of peroxides in the presence of lipids without the need for extraction steps.
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FEATURES
- Colorimetric Assay
- Linear Range: 0-50µM
- Highly Reproducible
- Enzyme Independent
- Rapid, Accurate & Inexpensive
APPLICATIONS
- Assay peroxide concentrations in biological solutions
- Measure lipid peroxidation
- Monitor protein glycation
References
- Bancells, C. et al (2009) J. Lipid Res. 50: 446-455