When recombinant proteins form inclusion bodies they cannot be used until the proteins in the inclusion bodies are solubilized in strong denaturing agents and then allowed to correctly fold into biologically active forms. Developing a folding protocol often requires a great deal of scientific literature research and experimentation.

The PROTEIN-Foldase™ kit is designed to simplify the optimization of protein folding procedures. This kit is based on a proprietary combination of denaturing agents for solubilization of inclusion bodies and the folding protocol based on rapid dilution of denatured protein into proprietary folding mixes.

Given the fact that every protein has unique folding properties, the kit offers a selection of five folding mixes. These folding mixes were developed using recognized compositions for protein folding that include the following folding agents:

•     Polyethylene Glycol
•     Cyclodextrine
•     ND SB-201 (non detergent sulfobetaine-201) detergent
•     Oxidizing agents
•     Reducing agents