TOTAL Arrest™
The TOTAL Arrest™ RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our proprietary binding matrix, pinkRESIN™. The absence of phenol/chloroform extractions make the Total Arrest™ RNA kit one of the safest methods for isolating and purifying high quality RNA. The 60 minute protocol (micro kit) is simple; after homogenization, RNA is bound to pinkRESIN™ and washed. The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from pinkRESIN™. The eluted RNA is ready for further applications, including Northern and dot blots, reverse transcription or RNase protective assays. The kit is supplied in two formats, the Micro for 50 preps (10-50mg tissue/ prep) and Large for 10 preps (100-500mg tissue/ prep).
Three step protocol:
-
Arrest™ Extraction Buffer lyses samples instantly destroying RNase activity.
-
pinkRESIN™ captures RNA.
-
After a brief washing step, pure and protein free RNA is eluted.
FEATURES
-
Rapidly inhibit destructive RNases
-
Strong chaotropic buffer for rapid RNA release
-
High affinity RNA binding matrix to capture RNA
-
Optional DNase treatment
-
Compatible with numerous species and tissues
-
Suitable for animal and plant tissues, cultured cells, blood and bacteria
-
Suitable for 50 x 50mg (Micro) or 10 x 500mg (Large) extractions
APPLICATIONS
- For the extraction and purification of protein and DNA free RNA.