Agarose I (General Purpose)

Protocol MSDS Certificate of Analysis

Catalog Description Size Price Action Shipping Condition Files
RC-122 Agarose I (All Purpose agarose) 5g $9.00 Room Temperature
RC-005 Agarose I (All purpose agarose) 25g $45.00 Not Updated
RC-006 Agarose I (All purpose agarose) 100g $133.00 Not Updated
RC-149 Agarose I (All purpose agarose) 500g $604.00 Not Updated

Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).

Specifications

Moisture	≤ 7%
Ash	≤ 4%
EEO	0.05-0.13
Sulfate	≤ 0.1%
Clarity 1.5% (NTU)	≤ 3
Gel Strength 1% (g/cm²)	≥ 1200
Gel Strength 1.5% (g/cm²)	≥ 2500
Gelling Temperature 1.5% (ºC)	36 ± 1.5
Melting Temperature 1.5% (ºC)	88 ± 1.5
DNase/ RNase Activity	None Detected
DNA Resolution ≥1000bp	Finely resolved
Gel background	Very low

Appropriate Agarose Concentrations

GEL CONCENTRATION (%)	1X TAE BUFFER	1X TBE BUFFER
0.6	1,000-20,000bp	1,000-15,000bp
0.8	500-12,000bp	500-10,000bp
1.0	300-8,000bp	250-7,000bp
1.2	200-6,000bp	200-5,000bp
1.5	100-3,500bp	100-3,000bp
2.0	50-2,000bp	50-2,000bp

FEATURES

Extraordinary mechanical resistance for more reliable and easier handling
Greater thermal stability due to high hysteresis (difference between gelling and melting temp)
High transparency and visibility
Easy preparation

APPLICATIONS

Analytical nucleic acid electrophoresis
Preparative nucleic acid electrophoresis
Blotting
Protein electrophoresis such as radial immunodiffusion

GbioSciences

GbioSciences

Agarose I (General Purpose)

GbioSciences

GbioSciences

Agarose I (General Purpose)

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