Agarose III (Pulse Field Gel Electrophoresis)
Agarose III has a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.
Specifications
|
Moisture |
≤ 7% |
|
Ash |
≤ 0.25% |
|
EEO (electroendosmosis) |
≤ 0.12 |
|
Sulfate |
≤ 0.12% |
|
Clarity 1.5% (NTU) |
≤ 4 |
|
Gel Strength 1% (g/cm2) |
≥ 1800 |
|
Gel Strength 1.5% (g/cm2) |
≥ 3200 |
|
Gelling Temperature 1.5% (ºC) |
36 ± 1.5 |
|
Melting Temperature 1.5% (ºC) |
88 ± 1.5 |
|
DNase/ RNase Activity |
None Detected |
|
DNA Resolution ≥1000bp |
Finely resolved |
|
Gel background |
Very low |
Appropriate Agarose Concentrations
| GEL CONCENTRATION (%) |
1X TAE BUFFER |
1X TBE BUFFER |
| 0.3 |
3,000-40,000bp |
2,000-20,000bp |
| 0.5 |
2,000-22,000bp |
1,500-12,000bp |
| 0.8 |
1,000-15,000bp |
1,000-9,000bp |
| 1.0 |
400-10,000bp |
500-6,000bp |
| 1.8 |
200-5,000bp |
200-3,000bp |
FEATURES
APPLICATIONS
- Pulse Field Gel Electrophoresis due to its higher exclusion limit.