Agarose III (Pulse Field Gel Electrophoresis)

Protocol MSDS Certificate of Analysis

Catalog Description Size Price Action Shipping Condition Files
RC-009 Agarose III (Pulse field gel electrophoresis) 25g $42.00 Ambient
RC-010 Agarose III (Pulse field gel electrophoresis) 100g $147.00 Ambient

Agarose III has a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.

Specifications

Moisture	≤ 7%
Ash	≤ 0.25%
EEO (electroendosmosis)	≤ 0.12
Sulfate	≤ 0.12%
Clarity 1.5% (NTU)	≤ 4
Gel Strength 1% (g/cm²)	≥ 1800
Gel Strength 1.5% (g/cm²)	≥ 3200
Gelling Temperature 1.5% (ºC)	36 ± 1.5
Melting Temperature 1.5% (ºC)	88 ± 1.5
DNase/ RNase Activity	None Detected
DNA Resolution ≥1000bp	Finely resolved
Gel background	Very low

Appropriate Agarose Concentrations

GEL CONCENTRATION (%)	1X TAE BUFFER	1X TBE BUFFER
0.3	3,000-40,000bp	2,000-20,000bp
0.5	2,000-22,000bp	1,500-12,000bp
0.8	1,000-15,000bp	1,000-9,000bp
1.0	400-10,000bp	500-6,000bp
1.8	200-5,000bp	200-3,000bp

FEATURES

High gel strength for lower gel concentrations for chromosomes and large sized particles like viruses and ribosomes
High electrophoretic mobility

APPLICATIONS

Pulse Field Gel Electrophoresis due to its higher exclusion limit.