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FOCUS™ Mammalian Proteome (6 Citations)

Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins.  Suitable for biological samples from animal tissues and adherent and suspension cells. 

 


Features

  • Single step extraction protocol.
  • Supplied with a strong proprietary chaotropic extraction buffer.
  • For 50 x 100mg animal tissue or 50μl wet cell pellet preparations.

 


Applications

  • Extraction of total proteins from mammalian tissues and cells.
  • Suitable for sample preparation for 2D gel electrophoresis and other applications.

 

 

Protocol
786-246
Material Safety Data Sheet
786-246
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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