Brij® 58 is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution.
Proteomic Grade: DG005, DG006, DG505, DG516
Research Grade: 786-522, 786-352
Proteomic Grade Detergent Solutions
Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds. The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and the carbonyl groups will form Schiff’s bases with the proteins that interfere with its structure.
G-Biosciences’ Proteomic Grade Detergent Solutions contain reduced peroxides and carbonyl compounds. In addition, the detergents have less than 50µS conductivity. These detergents are offered as 10% aqueous solutions, sealed under inert gas, and are suitable for protein applications. These non-ionic detergents are suitable for isolating membrane-protein complexes.
Other Proteomic Grade Detergent Solutions offered include: Triton® X-100, Triton® X-114, Brij® 35, Tween® 20, Tween® 80 and Nonidet® P-40 Substitute. A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic grade detergents, as well as one gram samples of one of our Zwitterionic detergents (CHAPS) and one of our non-detergent sulfobetaines (NDSB 201).
- Synonym: Polyethylene glycol hexadecyl ether
- CAS Number: 9004-95-9
- Form: White solid (unless indicated)
- Molecular Weight: 1122
- Absorbance (225nm): 0.0788 (1% w/v)
- Critical Micelle Concentration (CMC): 7-77µM
- Aggregation Number: 70
- Cloud Point: >100ºC
- Average Micellar Weight: 79,000
- Molecular Formula: C16H33(OCH2CH2)20-OH
- Non-ionic detergent available as a 10% proteomic grade solution or a research grade solid
- For protein extraction, permeabilization of cells, and preparation of yeast spheroplasts
- Ultra low concentrations of contaminating peroxides, aldehydes, salts and carbonyl compounds
- For more information on the properties and uses of detergents in research and for further information on our detergent removal systems, view our Detergents Handbook: a handbook and selection guide for proteomic grade detergents, research grade detergents, non-ionic, ionic and Zwitterionic detergents, detergent estimation, and detergent removal systems.