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Protein-Free Blocking Buffer (2 Citations)




Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications.  The absence of protein from the Protein-Free Blocking Buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins. 

 

Protein-Free Blocking Buffer eliminates any concern associated with regulatory compliance issues where use of animal source components are restricted. Furthermore, Protein-Free™ Blocking Buffer is compatible with antibodies and avidin/biotin based systems and results in high signal to background ratios.

 

For user’s convenience Non-Protein Blocking Buffers are supplied in widely used TBS (Tris-buffered saline at pH 7.5) and PBS buffers (phosphate-buffered saline at pH 7.5) as well as in separate formulations containing Tween®-20 for improving blocking efficiencies.

 


Features

  • Protein free blocking agent
  • Eliminate or minimize cross reactivity with animal source antibodies and antigens
  • High signal to background ratios
  • Four convenient formats, with and without detergent
  • Ready-to-use

 

 

Protocol
786-662
786-663
786-664
786-665
Material Safety Data Sheet
786-662
English_US
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English_UK
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786-663
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-664
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-665
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Bioassays Handbook
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
Certificate Of Analysis
786-665
203010

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