Southern blot analysis, named after its inventor Edwin M. Southern, is a common research technique for enhancing the results of agarose electrophoresis. DNA is resolved on an agarose gel and is subsequently transferred to a nylon membrane.
The resulting membrane can be probed for specific DNA sequences using a single stranded probe that anneals or hybridizes with a specific sequence. The hybridization probes are often labeled, which can be detected later.
The Southern Blot Analysis kit is a class demonstration kit that allows students to visualize the Southern blot technique and visualize the blotted DNA with a safe blue stain.
Supplied with all reagents needed for a class demonstration, including a detailed Teacher’s Guide.
Applications
- Class demonstration of Southern blot technique.
- Demonstrate agarose electrophoresis.
- Introduce principle of probes and hybridization.
