UPPA-I & II Pack (54 Citations)
Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents, chaotropics, or other common laboratory agents. The protein precipitate is suspended in a small volume buffer. If the protocol is followed correctly, the recovery is generally 100%.
UPPA™ is suitable for concentrating proteins for running gels, protein purification, protein assays, and other applications. It is not suitable for proteins which may lose some of their biological activities when precipitated; for such proteins, use our Column-PROTEIN-Concentrate™ (Cat. No. 786-126) or our OrgoSol-PROTEIN-Concentrate™ (Cat. No. 786-125).
Applications
- Concentrate proteins for 1D electrophoresis, protein purification and protein assays
Protocol | |
786-122 |
Material Safety Data Sheet | |
786-122 |
Technical Literature | |
Concentration of Dilute Protein Solutions | |
Mass Spectrometry Sample Prep Handbook | A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation. |
Plant Proteomics Handbook | |
Protein Electrophoresis Handbook | A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products. |
Protein Labeling & Conjugation | |
Sample Preparation Handbook | For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment |
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