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Well-Coated™ Sulfhydryl Binding



Well-Coated™ Sulfhydryl Binding plates are designed to specifically bind free sulfhydryls of peptides, proteins and other molecules.  The Well-Coated™ Sulfhydryl Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for binding assays.

 

Well-Coated™ Sulfhydryl Binding plates are maleimide activated plates that react with free sulfhydryls to form stable thioether bonds at pH 6.5-7.5.  pH >7.5 significantly increases the reaction of amines with the maleimide groups. 

 

The wells are coated to a 100µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer.  The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.

 

We recommend the use of  Ellman’s Reagent  to determine the amount of free sulfhydryls present on your peptdie, protein or ligand. For reducing agents to reduce disulfide bridges to generate free sulfhydryls click here.  For peptides or proteins lacking sulfhydryls, SATA (N-Succinimidyl-S-acetylthioacetate) or Traut’s Reagent (2-Iminothiolane hydrochloride) can be used to add sulfhydryls via amine modification.

 

 


Features

 


Applications

  • For the binding of peptides, proteins and other ligands through free sulfhydryls.

 

 

Protocol
786-755
786-780
786-781
Material Safety Data Sheet
786-755
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786-780
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-781
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Bioassays Handbook

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