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FOCUS™ Yeast Proteome

Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins.  Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilizes even the most difficult proteins.

 


Features

  • Simple extraction protocol.
  • Supplied with yeast specific lytic enzyme preparation.
  • Supplied with a strong proprietary chaotropic extraction buffer.
  • Suitable for 50 x 60μl yeast cell pellet preparations.

 


Applications

  • Extraction of total proteins from yeast cells.
  • Suitable for sample preparation for 2D gel electrophoresis and other applications.

 

 

Protocol
786-257
Material Safety Data Sheet
786-257
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment

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