The TPE™ kit is a universal lysis system designed for the solubilization and extraction of total proteins from cells and tissues for SDS-PAGE Analysis. The kit is suitable for animal, plant, yeast, bacteria, and other biological samples.
The TPE™ kit provides a two component protocol that eliminates clump formation, protein loss, and other problems associated with total protein extraction procedures. Samples are ground in the buffer provided and then heated to solubilize the total protein.
The TPE™ kit is based on optimized concentration of Tris and SDS and is suitable for running denaturing electrophoresis and other downstream applications.
FEATURES
- Ready-to-use buffers
- Two component extraction protocol
- Based on an optimized concentration of Tris and SDS
- Supplied with sufficient reagents for 50 x 250mg preparations
APPLICATIONS
- For solubilization of total proteins for electrophoresis and other applications
Technical
- For more information on 1D and 2D electrophoresis and electrophoresis products, view our Protein Electrophoresis Handbook: a handbook and selection guide for protein gel preparation, protein markers, electrophoresis buffers, reducing and alkylating reagents, electrophoresis products and accessories, protein gel stains, clean-up and concentration systems, high abundance protein removal, protein fractionation and enrichment, and protein extraction and lysis systems.
- For more information on preparing protein samples, view our Sample Preparation (Lysis, Fractionation, Clean-Up & Concentration) Handbook: a handbook and selection guide for lysis buffers and systems, protein fractionation kits, dialysis systems, electrophoresis clean-up and concentration systems, and contamination removal kits.
- For more information on Western blots, electrophoresis and accessories, view our Western Blotting & Electrophoresis Handbook: a handbook and selection guide for 1-hour Western systems, transfer buffers and membranes, membrane stains, stripping solutions, detection reagents, re-probing reagents, protein electrophoresis, blocking agents, secondary antibodies, protein extraction and lysis, washing buffers, and troubleshooting.
- For more information on the preparation of protein samples for mass spectrometry and the products offered, view our Mass Spectrometry Sample Prep Handbook: a handbook and selection guide for protein sample preparation, protein clean-up systems, electrophoresis reagents, mass spec grade protease, in-gel digestion kits, and peptide generation reagents.