Western ReProbe™ is a single component system, specifically formulated to dissociate and remove antibodies from membrane bound proteins without destroying the antigenic binding affinity and does not use denaturants, SDS or boiling. Western ReProbe™ allows you the ability to reuse your Western blots.
Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).
Western ReProbe™ is not recommended for stripping color producing Western blots that use substrates such as TMB, chloronapthol and DAB.
Western ReProbe™ is supplied as a 5X solution; sufficient reagents for 25-30 standard (7.5 x 8.5cm) Western blots.
Additional Images
figure-1
FEATURES
Advantages of Western ReProbe™
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Repeated analysis of Western blots with multiple probes.
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Re-analysis and correction of unsatisfactory Western blots.
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Conservation of hard-to-obtain test samples and reagents.
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Time saving in not rerunning gels and transfer processes.
Western ReProbe™ is supplied as a 5X solution; sufficient reagents for 25-30 standard (7.5 x 8.5cm) Western blots.
APPLICATIONS
- Screen same blot with different antibodies.
- Reprobe for housekeeping proteins.
- Compare phosphorylated and total protein on the same blot.
References
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Di, X. et al (2008) Hum. Reprod. 23: 1873-83.
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Roy, S. and Tenniswood, M. (2007) J. Biol. Chem. 282: 4765.
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George, J. et al (2007) Clin Cancer Res. 13(12): 3507-17.
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Joshi, M. et al (2006) Amer J. Respir. 10.1164.
- Bu, S. et al (2006) Reproduction 131:1099.
- Lam, A.M.I and Frick, D.N. (2006) J. Virol. 80: 404.
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Roy, S. et al (2005) Cell Death Differ. 12: 482.