Western ReProbe™ is a single component system, specifically formulated to dissociate and remove antibodies from membrane bound proteins without destroying the antigenic binding affinity and does not use denaturants, SDS or boiling. Western ReProbe™ allows you the ability to reuse your Western blots.
Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).
Western ReProbe™ is not recommended for stripping color producing Western blots that use substrates such as TMB, chloronapthol and DAB.
Western ReProbe™ is supplied as a 5X solution; sufficient reagents for 25-30 standard (7.5 x 8.5cm) Western blots.
Advantages of Western ReProbe™
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Repeated analysis of Western blots with multiple probes.
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Re-analysis and correction of unsatisfactory Western blots.
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Conservation of hard-to-obtain test samples and reagents.
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Time saving in not rerunning gels and transfer processes.
Western ReProbe™ is supplied as a 5X solution; sufficient reagents for 25-30 standard (7.5 x 8.5cm) Western blots.