The XIT^™ Genomic DNA from Gram Negative Bacteria kit is designed for the isolation of genomic DNA from overnight cultures.

 

The kit uses three main steps (see figure) that are rapid & efficient bacterial lysis, complete protein precipitation, followed by DNA precipitation.  This yields high quality genomic DNA.

 

Two sizes of kit are available for processing a total of 25 and 250ml of bacterial culture.  XIT^™ Genomic DNA from Gram Negative Bacteria Kit protocol is designed to use 1ml overnight culture, however the protocol can be easily adapted for larger tissue sample sizes. The purified DNA has a A₂₆₀/A₂₈₀ ratio between 1.7 and 1.9 and is up to 200kb in size.  The yield is approximately 20-40µg/ml culture.