The XIT^™ Genomic DNA from Gram Positive Bacteria kit is designed for the isolation of genomic DNA from overnight cultures.

 

The kit uses three main steps (see figure) that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation.  This yields high quality genomic DNA.

 

Two sizes of kit are available for processing a total of 25 and 250ml of bacterial culture.  The purified DNA has a A₂₆₀/A₂₈₀ ratio between 1.7 and 1.9 and is up to 200kb in size.  The yield is approximately 15-25µg/ml culture.