The XIT™ Genomic DNA from Plant Tissue kit is designed for the isolation of genomic DNA from fresh or frozen plant tissues.
The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
Two sizes of kit are available for processing a total of 2.5 and 25g of tissue. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 1-5µg/mg tissue, dependent on plant species.
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FEATURES
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Isolate high quality genomic DNA
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Suitable for fresh, frozen and fixed tissue
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Yield 0.5-10µg/mg tissue
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Suitable for archive quality DNA.