Yeast PE LB™ kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast PE LB™ is based on organic buffering agents and utilizes a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins.
A ready-to-use Zymolyase® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Yeast PE LB™. Yeast PE LB™ has been tested on several widely used yeast strains.
The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LB™ eliminates the need for laborious glass bead lysis of yeast cells.
This kit is suitable for processing 100 x 50µl yeast cell pellet preparations. Yeast PE LB™ buffer is also available separately for further downstream processing.
FEATURES
- Eliminates the need for glass bead lysis.
- Supplied as a kit, containing Zymolyase® and reagents needed for protein extraction.
- Suitable for processing 100 x 50µl yeast cell pellet preparations.
APPLICATIONS
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Lysis and extraction of proteins from yeast cells.
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Isolation of spheroplasts.
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For the isolation of biologically active proteins.
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Suitable for most applications including enzyme and protein purifications, electrophoresis, Western blotting and 2D-gel analysis.
References