femtoCHROMO™-HRP kit is supplied with a single step chromogenic substrate for the rapid development of protein bands with low background staining.  To ensure low background staining, each kit is supplied with an enhanced blocking agent, BLOT-QuickBlocker™, and a concentrated (10X) washing buffer, femtoTBST Buffer.

 

In addition, optional HRP or AP labeled secondary antibodies against mouse or rabbit antibodies are supplied.

 

femtoCHROMO™-HRP kit contains a modified TMB (3, 3', 5, 5'-Tetramethylbenzidine) substrate that generates a dark blue precipitate in the presence of horseradish peroxidase.