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femtoLUCENT™ PLUS


Quick and sensitive non-isotopic detection of proteins on transfer membranes.
 
A femtogram level sensitive immunodetection system allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1,2 dioxetane supersensitive detection reagent and a unique, proprietary combination of blocking agent (NAP-BLOCKER™) and washing buffers to ensure low background and high signal to background ratio. Optional horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat are also offered for a complete chemiluminescence detection system.

femtoLUCENT™ PLUS is offered as a complete immunodetection system with all of the critical reagents needed for Western blots or dot-blot analysis or as a reagent kit for those who prefer to customize their detection methods with the choice of their own secondary antibody conjugates.

femtoLUCENT™ PLUS Reagent kit includes

  • Detection reagents for HRP or AP
  • NAP-BLOCKER™, a non animal protein blocking agent
  • femto-TBST™ washing buffer
  • Optional secondary antibodies
Additional Images

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FEATURES

    All of the critical reagents provide:

    • Economical: Greater value compared to similar products.
    • Intense light emission with low background : high signal ratio.
    • Low femtogram detection (10-15), allows detection of >10fg protein on a dot blot and >1pg on a Western blot.
    • Supplied with a novel blocking agent which allows a rapid blocking step and produces a clear background.
    • Suitable for nitrocellulose & PVDF membranes.

     

APPLICATIONS

    • Immunodetection of proteins and antigens on Western blots and dot blot applications.


     

References
    1. Van Zandt, K. et al (2008) J. Leukoc. Biol. 84: 689-700.
    2. Zandt, K. et al (2008) J. leukoc. Biol.  84: 689-700.
    3. Gopalakrishnan, R. and Chandra, N.C. (2006) Ind. J. Clin. Biochem. 21: 8.
    4. Fenton, J. et al (2006) Carcinogenesis 27: 1507.
    5. Wang, Y. et al (2005) J. Immunol. 174: 5687.
    6. Fenton, J. et al (2005) Cancer Epid. 14: 1646. 

MSDS   
  • Catalog
  • Description
  • Size
  • Price
  • Action
  • Shipping Condition
  • Protocol
  • 786-10APT
  • femtoLUCENT™ PLUS AP substrate only
  • 300cm2
  • $74.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R46
  • femtoLUCENT™ PLUS AP with AP-goat α-human antibody
  • 1500cm2
  • $338.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R43
  • femtoLUCENT™ PLUS AP with AP-goat α-mouse antibody
  • 1500cm2
  • $343.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R44
  • femtoLUCENT™ PLUS AP with AP-goat α-rabbit antibody
  • 1500cm2
  • $343.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R45
  • femtoLUCENT™ PLUS AP with AP-goat α-rat antibody
  • 1500cm2
  • $343.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R47
  • femtoLUCENT™ PLUS AP with AP-rabbit α-goat antibody
  • 1500cm2
  • $338.00
  • Add to Cart
  • Ambient
  • View
  • 786-10AP-R49
  • femtoLUCENT™ PLUS AP with AP-rabbit α-human antibody
  • 1500cm2
  • $327.00
  • Add to Cart
  • Ambient
  • View
  • 786-10T
  • femtoLUCENT™ PLUS HRP substrate only
  • 1000cm2
  • $29.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R41
  • femtoLUCENT™ PLUS HRP with HRP-goat α-human antibody
  • 5000cm2
  • $382.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R38
  • femtoLUCENT™ PLUS HRP with HRP-goat α-mouse antibody
  • 5000cm2
  • $298.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R39
  • femtoLUCENT™ PLUS HRP with HRP-goat α-rabbit antibody
  • 5000cm2
  • $298.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R40
  • femtoLUCENT™ PLUS HRP with HRP-goat α-rat antibody
  • 5000cm2
  • $382.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R42
  • femtoLUCENT™ PLUS HRP with HRP-rabbit α-goat antibody
  • 5000cm2
  • $389.00
  • Add to Cart
  • Ambient
  • View
  • 786-10-R48
  • femtoLUCENT™ PLUS HRP with HRP-rabbit α-human antibody
  • 5000cm2
  • $384.00
  • Add to Cart
  • Ambient
  • View