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femtoLUCENT™ PLUS
Quick and sensitive non-isotopic detection of proteins on transfer membranes.
A femtogram level sensitive immunodetection system allows users the option to detect even hard-to-detect low abundance proteins. This immunodetection system is based on an innovative formulation of luminol and 1,2 dioxetane supersensitive detection reagent and a unique, proprietary combination of blocking agent (NAP-BLOCKER™) and washing buffers to ensure low background and high signal to background ratio. Optional horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibodies against mouse, rabbit, human, rat and goat are also offered for a complete chemiluminescence detection system.
femto
LUCENT™ PLUS is offered as a complete immunodetection system with all of the critical reagents needed for Western blots or dot-blot analysis or as a reagent kit for those who prefer to customize their detection methods with the choice of their own secondary antibody conjugates.
femto
LUCENT™ PLUS Reagent kit includes
Detection reagents for HRP or AP
NAP-BLOCKER™, a non animal protein blocking agent
femto
-TBST™ washing buffer
Optional secondary antibodies
Additional Images
figure-1
figure-2
FEATURES
All of the critical reagents provide:
Economical: Greater value compared to similar products.
Intense light emission with low background : high signal ratio.
Low femtogram detection (10
-15
), allows detection of >10fg protein on a dot blot and >1pg on a Western blot.
Supplied with a novel blocking agent which allows a rapid blocking step and produces a clear background.
Suitable for nitrocellulose & PVDF membranes.
APPLICATIONS
Immunodetection of proteins and antigens on Western blots and dot blot applications.
References
Van Zandt, K. et al (2008) J. Leukoc. Biol. 84: 689-700.
Zandt, K. et al (2008) J. leukoc. Biol.
84: 689-700.
Gopalakrishnan, R. and Chandra, N.C. (2006) Ind. J. Clin. Biochem. 21: 8.
Fenton, J. et al (2006) Carcinogenesis 27: 1507.
Wang, Y. et al (2005) J. Immunol. 174: 5687.
Fenton, J. et al (2005) Cancer Epid. 14: 1646.
MSDS
Catalog
Description
Size
Price
Action
Shipping Condition
Protocol
786-10AP
femto
LUCENT™ PLUS AP
1500cm
2
$180.00
Add to Cart
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786-10APT
femto
LUCENT™ PLUS AP substrate only
300cm
2
$74.00
Add to Cart
Ambient
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786-10AP-R46
femto
LUCENT™ PLUS AP with AP-goat α-human antibody
1500cm
2
$338.00
Add to Cart
Ambient
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786-10AP-R43
femto
LUCENT™ PLUS AP with AP-goat α-mouse antibody
1500cm
2
$343.00
Add to Cart
Ambient
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786-10AP-R44
femto
LUCENT™ PLUS AP with AP-goat α-rabbit antibody
1500cm
2
$343.00
Add to Cart
Ambient
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786-10AP-R45
femto
LUCENT™ PLUS AP with AP-goat α-rat antibody
1500cm
2
$343.00
Add to Cart
Ambient
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786-10AP-R47
femto
LUCENT™ PLUS AP with AP-rabbit α-goat antibody
1500cm
2
$338.00
Add to Cart
Ambient
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786-10AP-R49
femto
LUCENT™ PLUS AP with AP-rabbit α-human antibody
1500cm
2
$327.00
Add to Cart
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786-10
femto
LUCENT™ PLUS HRP
5000cm
2
$180.00
Add to Cart
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786-10T
femto
LUCENT™ PLUS HRP substrate only
1000cm
2
$29.00
Add to Cart
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786-10-R41
femto
LUCENT™ PLUS HRP with HRP-goat α-human antibody
5000cm
2
$382.00
Add to Cart
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786-10-R38
femto
LUCENT™ PLUS HRP with HRP-goat α-mouse antibody
5000cm
2
$298.00
Add to Cart
Ambient
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786-10-R39
femto
LUCENT™ PLUS HRP with HRP-goat α-rabbit antibody
5000cm
2
$298.00
Add to Cart
Ambient
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786-10-R40
femto
LUCENT™ PLUS HRP with HRP-goat α-rat antibody
5000cm
2
$382.00
Add to Cart
Ambient
View
786-10-R42
femto
LUCENT™ PLUS HRP with HRP-rabbit α-goat antibody
5000cm
2
$389.00
Add to Cart
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786-10-R48
femto
LUCENT™ PLUS HRP with HRP-rabbit α-human antibody
5000cm
2
$384.00
Add to Cart
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