The pinkCLEANUP™ kit uses pinkRESIN™ to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparations of 100bp to >20kb. pinkRESIN™ has an enhanced binding affinity for DNA and resuspends with ease, thus eliminating loss or damage of DNA; a major frustration of using binding matrix for isolation of DNA.
The DNA cleaning protocol takes as little as 5 minutes:
1. Bind DNA to pinkRESIN™.
2. Wash away primers, enzymes, salts, and other impurities.
3. Elute clean DNA.
Optional spin columns are also offered to simplify the procedure further. pinkRESIN™ is also offered separately for those who prefer to customize their own protocol.
FEATURES
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pinkRESIN™ has enhanced DNA binding affinity and rapidly resuspends
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Protocol is 5-10 minutes.
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No toxic phenol/chloroform extraction.
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No alcohol precipitations.
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Optional spin columns.
APPLICATIONS
- Applications include PCR clean-up, purifying DNA fragments after in-vitro labeling reactions, removal of restriction enzymes from plasmid DNA prior to in-vitro. transcription, or cleaning any DNA preps