Polyacrylamide gel electrophoresis (PAGE) is regularly used in protein research to separate a mixture of proteins by their molecular weight. Protein electrophoresis uses polyacrylamide gels that are made, or cast, from a mixture of acrylamide and bisacrylamide that is cross-linked by the action of the catalysts TEMED and ammonium persulfate.
Acrylamide/ Bisacrylamide (19:1) Mix: a premixed mixture of acrylamide and bisacrylamide at 19:1 ratio that will produce ideal cross-linked gels upon polymerization. Using the premixed powder minimizes the risk associated with handling this neurotoxin.
Ammonium Persulfate (APS and Temed): Act together to initiate and catalyze the polymerization of the acrylamide and bisacrylamide.
Electrophoresis Running Buffer (10X): A 10 times concentrated (10X) (Tris, Glycine & SDS) buffer for performing protein electrophoresis. Simply dilute the solution (1:10) in water and use.
Sample Loading Buffer (2X): A sample loading buffer suitable for protein electrophoresis. Simply add an equal volume to the protein sample, boil and load the sample. Reducing agents, such as b-mercaptoethanol, can be added if required.
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-Mercaptoethanol: A common reducing agent used to destroy disulfide bonds in proteins before electrophoresis.
Protein Marker: Unstained protein standard markers of 66, 29, 17, 14 and 6.5kDa molecular weight. Suitable for determining the molecular weight of unknown proteins.
Protein Gel Stain: LabSafe GEL Blue™ is an enhanced protein gel stain, which is based on Coomassie dye and offers unsurpassed sensitivity and rapid protein band visualization. It is supplied in a ready-to-use format, which is added directly to protein gels following electrophoresis after a brief wash step.