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2x YT Broth

2x_YT_Broth

2xYT Broth is a microbial growth medium commonly used for cultivation of E. coli and M13 bacteriophage. The 2x YT medium is rich in nutrients such as peptides, amino acids, and water-soluble vitamins and has low concentration of salts.

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Features:

  • Composition: 1.6 % Tryptone, 1% Yeast Extract and 0.5 % sodium chloride.
  • Sterile filtered using 0.2 μ filter unit.
  • pH is adjusted to 6.8±0.2
  • available in 1 L and 10 x 1 L sizes.

Application(s)

  • Suitable for cultivation of M13 bacteriophage.
  • Suitable for cultivation of E.coli for cloining, DNA plasmid production and production of recombinant proteins

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