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Boronate Resin





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Our Boronate resin is designed for the isolation of ribonucleotide and oligonucleotide RNA.

 

The resin consists of boronic acid covalently linked to a polyacrylamide support that offers simple isolation of small molecular weight compounds that have cis-diol groups.

 

Mechanism: The boronic acid interacts with the cis-diol groups, found in the sugar portion of nucleotides, forming a reversible five member ring complex. Impurities are washed away and then the complex dissociated by low pH or presence of sorbitol.

 

The polyacrylamide support excludes >6000 Da molecules from entering the resin bed and therefore is suitable only for small molecules.

 


Features

  • Polyacrylamide gel
  • >1.2meq/g boronate load capacity
  • >130µmol sorbitol/ml gel binding capacity
  • 6,000Da exclusion limit
  • 45-90µm wet bead size

 

 

Protocol
786-314
786-315
786-317
786-823
786-313
Material Safety Data Sheet
786-314
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786-315
English_US
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786-317
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786-823
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
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Swedish
Polish
786-313
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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