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Affinity Purification Resins

Download the Protein Purification Handbook for a full list of related productsDownload the Purification Resins Handbook for Complete Product List

 

For the separation & purification of affinity tagged proteins & antibodies
 
The separation and purification of proteins has been a major challenge for researchers in the early days of proteomic research. The advances in affinity chromatography have enabled researchers to purify large quantities of highly pure proteins for a multitude of analysis techniques, including crystallography, protein:protein studies and in-vitro assays.
Affinity chromatography works by binding a protein, via a reversible interaction, to a specific ligand that is prebound to a solid chromatographic support. The protein(s) are first bound to the column in a buffer that supplies conditions optimal for binding. Unbound, non-specific material is washed away and the protein(s) of interest are then eluted by changing the buffering conditions to induce desorption from the solid support.
We supply two main groups of affinity resins. The first group is for the separation and purification of affinity tagged proteins and the second group is for the binding of immunoglobulin molecules.
A common practice in today’s research is the use of molecular biology to clone our protein(s) of interest into a vector that adds a specific tag to the protein. The most versatile and common tags used are glutathione S-transferase (GST), a 6x histidine motif (His-tag), and the calmodulin binding peptide (CBP).
EndotoxinOUT™ was designed for the rapid removal of endotoxins and pyrogens (LPS, lipopolysaccharides) from nucleic acid (DNA) samples. EndotoxinOUT™ is an ideal product for the cleanup of buffers, cell culture media, protein solutions, nucleic acid (DNA) samples and pharmacological comp..
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FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.   FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Co IDA Agarose Fast Flow is specifically designed fo..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™ Ni IDA Agarose Fast Flow is specifically designed fo..
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals.  G-Sep™NI NTA Agarose Fast Flow is specifically designe..
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G-Trap™ Co IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).   IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein residues h..
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G-Trap™ Ni IDA Agarose Fast Flow columns are ready to use prepacked affinity columns used for purification of proteins based on immobilized metal ion affinity chromatography (IMAC).   IMAC is based on interaction of cations of transition metals such as Ni2+, Co2+ with protein resid..
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Model:
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin. Su..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from bacterial cultures. The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the structure and activity o..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of ..
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HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from bacterial cultures.    The bacteria are first lysed with Bacterial PE LB™ and PE LB™-Lysozyme to release total soluble protein, whilst maintaining the str..
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HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and a..
$0.00
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