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No-Waste™ Freund's Incomplete Adjuvant (FIA)

Catalog Description Size Price(USD) Qty
786-098
No-Waste™ Freund's Incomplete Adjuvant 2ml
$18.00
786-099
No-Waste™ Freund's Incomplete Adjuvant 5 x 2ml
$49.00

No-Waste™ Freund's Incomplete Adjuvant (FIA)

Our No-Waste™ format adjuvant minimizes waste and risk of cross contamination.  Small dose packaging allows researchers to assign a vial per project or animal without concerns for excessive documentation and monitoring.  Researchers do not have the burden of storing large amounts of unused adjuvant.

 

Freund’s Incomplete Adjuvant, also known as Incomplete Freund's Adjuvant (IFA), lacks the heat-killed mycobacteria present in Freund’s Complete Adjuvant.

 

Freund’s Incomplete Adjuvant comprise of non-metabolizable oil, paraffin and the surfactant mannide monooleate. The mineral oil used in Freund’s Adjuvant has depot effect on antigen as it is retained and released slowly at the site of injection and thus provide continuous access of antigen to immune system to raise antibodies.Retention of antigen also helps in presentation of antigen to antigen presenting cells, macrophages and dentritic cells and thus promoting cell mediated immune response.  Mineral oil also act as a vehicle to transport antigen in lymphatic system.

 

Freund’s Incomplete adjuvant is used along with Freund’s Complete Adjuvant to raise polyclonal and /or monoclonal antibodies. Freund’s Complete Adjuvant is used in primary immunization where as Freund’s Incomplete Adjuvant is used in secondary and booster injections. Freund’s Incomplete Adjuvant has less side affects compare to Freund’s Complete Adjuvant. Freund’s Complete Adjuvant form granulomas which may result in inflammation and lesions. Freund’s Incomplete Adjuvant can be used for primary immunization if the antigen is strongly immunogenic.

 


Features

  • No-Waste™ format: Small volume size eliminates wastage and cross contamination
  • Water-in-oil emulsion without Mycobacteria
  • Enhnaces immune response to antigen
  • Used for booster injecitons

 


Applications

  • It is used as water in oil emulsion with antigen for primary immunization to raise polyclonal and monoclonal antibodies for research purposes

 

 

Protocol
786-098
786-099
Material Safety Data Sheet
786-098
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English_UK
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786-099
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
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    References

    1. Freund, J. and McDermott, K. (1942) Proc. Soc. Exp. Biol. Med. 49, 548-553
    2. Freund, J. (1947) Ann. Rev. Microbiol. 1, 291
    3. Freund, J. (1956) Adv. Tuberc. Res. 7,130 
    4. Herbert, W.J. (1967. In Symposium Series of Immunobiology Standardization. Vol.6  Basel  NY: Karger. p213-220
    5. Male, D., Champion, B. and Cooke, A. (1987). Advanced Immunology. J.B. Lippincott Co., Gower Medical publishing, London 8.1-8.8
    6. Harlow, E. and Lane, D. (1998) Antibodies A Laboratory Manual.  Cold Spring Harbor laboratory, Cold Spring Harbor, New York 56-100
    7. Deeb, B. J. et al. (1992) J. Immuno. Meth. 152 , 105-113
    8. Bennet, B. et al. (1992) J. Immuno. Meth. 153 31-40


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